equipment and reagents for dna extraction of plant materials

Evaluation of Synergy Plant DNA Extraction Chemistry

Evaluation of Synergy Plant DNA Extraction Chemistry

Evaluation of the Synergy Rapid Plant DNA Isolation Chemistry Fiona K. Fitzgerald and David W. Burden OPS Diagnostics LLC, P.O. Box 348, Lebanon, NJ 08833 This research paper is available as a PDF through this link. Abstract A new chemistry (Synergy Plant DNA Extraction Kit) for the isolation of DNA from plant tissues was evaluated and compared to traditional CTAB and DNeasy® methods.

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Evaluation of Synergy Plant DNA Extraction Chemistry

Evaluation of Synergy Plant DNA Extraction Chemistry

Evaluation of the Synergy Rapid Plant DNA Isolation Chemistry Fiona K. Fitzgerald and David W. Burden OPS Diagnostics LLC, P.O. Box 348, Lebanon, NJ 08833 This research paper is available as a PDF through this link. Abstract A new chemistry (Synergy Plant DNA Extraction Kit) for the isolation of DNA from plant tissues was evaluated and compared to traditional CTAB and DNeasy® methods.

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DNeasy Plant Pro Kit

DNeasy Plant Pro Kit

 · DNA was isolated from 50 mg of plant material (4 replicates each) using the DNeasy Plant Pro, DNeasy Plant Mini and DNeasy PowerPlant Pro Kits. DNA yields were quantified with a Qubit Fluorometer. (A) Plants with high DNA content (grass and pine needles); (B) Plants with lower DNA content (old strawberry, mint or citrus (lemon) leaf).

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Toxic reagents and expensive equipment: are they really …

Toxic reagents and expensive equipment: are they really …

Biological materials tested for DNA extraction were prepared as follows: Mycelium 10 ml of Malt Extract Broth (MEB: Malt Extract 20 g l −1, Glucose 20 g l −1, Peptone 1 g l −1 ) (in a 50 ml Falcon tube) were inoculated with a loop full of spores and incubated for 72 h at 25°C, in the dark, with slight agitation.

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Plant/Fungi DNA Isolation Kit | Sigma-Aldrich

Plant/Fungi DNA Isolation Kit | Sigma-Aldrich

Customer-Supplied Reagents and Equipment You must have the following in order to use the Plant/Fungi DNA Isolation Kit: Benchtop microcenrifuge 65 C Incubator Ice bath 96-100 % ethanol 70 % ethanolv Liquid nitrogen or any mechanical homogenizer

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Extracting DNA from living things

Extracting DNA from living things

You can extract DNA – to see what it is like – from some plant and some animal material using equipment and chemicals you might find in a kitchen. For more thorough analytical work, you need more control over the components of your chemicals, and it may be …

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Specialised kits for isolating DNA from plant materials such as plant leaf, seeds, roots and stem. View Exgene Plant SV GeneAll View GenEx Plant & GenEx Plant Plus kit GeneAll View QuickExtract Plant DNA Extraction Solution BioSearch Tech

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Extraction of DNA From Plants Using Plant DNAzol …

Extraction of DNA From Plants Using Plant DNAzol …

Plant DNAzol ® is an extra-strength-DNAzol ® reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plant DNAzol ® procedure is based on the use of a novel guanidine-detergent lysing solution which hydrolyzes RNA and …

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An easily-performed high-throughput method for …

An easily-performed high-throughput method for …

 · Here we develop a plant gnomic DNA extraction method based on the traditional approach of cetyl-trimethyl ammonium bromide (CTAB) extraction. This protocol uses YM-CTAB DNA isolation buffer, with 2 mL microcentrifuge tubes each containing two 3 mm stainless steel balls for sample collection and grinding; DNA is then purified in 1.5 mL microcentrifuge tubes, effectively avoiding …

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An Efficient Multistrategy DNA Decontamination …

An Efficient Multistrategy DNA Decontamination …

 · (4) Contamination of PCR reagents and DNA extraction kits with bacterial DNA is a major problem when broad-range primers are used for the detection in clinical specimens of bacterial consensus DNA sequences, such as bacterial 16S DNA, (e.g., may be

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DNA EXTRACTION METHODS

DNA EXTRACTION METHODS

Non-Organic DNA Extraction •Does not use organic reagents such as phenol or chloroform. •Digested proteins are removed by salting out with high concentrations of LiCl. •However, if salts are not adequately removed, problems could occur with the Dr.L

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innuPREP Plant DNA Kit | Analytik Jena

innuPREP Plant DNA Kit | Analytik Jena

The innuPREP Plant DNA Kit has been specially developed for quickly and easily isolating DNA from an extremely wide variety of plant starting materials (such as leaves, stems, roots, flowers, etc.). In addition to efficient sample digestion, the extraction routine also includes a prefiltration step to effectively minimize unlysed plant components. The DNA is then bound to a Spin Filter column ...

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Plant DNA Extraction Protocol

Plant DNA Extraction Protocol

Extraction Protocol 1. Weight out 0.3 g of plant tissue 2. Place tissue on a clean glass slide. Chop the tissue into a paste using a clean single edge razor blade. (we have also modified a Dremel Roto-tool for use as a simple tissue homogenizer with good

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Enhanced Protocol for Isolation of Plant Genomic DNA

Enhanced Protocol for Isolation of Plant Genomic DNA

Several plant DNA extraction protocols have been used and compared to find the best protocol that isolates high-quality, amplifiable DNA. During the extraction, the aim is to lessen the effect of

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Veterinary Lab Workflows

Veterinary Lab Workflows

The innuPREP Plant DNA Kit has been specially developed for quickly and easily isolating DNA from an extremely wide variety of plant starting materials (such as leaves, stems, roots, flowers, etc.). In addition to efficient sample digestion, the extraction routine also includes a prefiltration step to effectively minimize unlysed plant components. The DNA is then bound to a Spin Filter column ...

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Quick-DNA Plant/Seed Miniprep Kit

Quick-DNA Plant/Seed Miniprep Kit

• DNA Yield – Typically 20-80 ng DNA/mg plant material. • DNA Purity – High quality, inhibitor-free DNA is eluted with DNA Elution Buffer and is suitable for PCR amplification (A 260 /A 280 > 1.8). procedures. Some reagents • DNA Size Limits – Capable of

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Extracting DNA from living things

Extracting DNA from living things

You can extract DNA – to see what it is like – from some plant and some animal material using equipment and chemicals you might find in a kitchen. For more thorough analytical work, you need more control over the components of your chemicals, and it may be …

Get price
DNeasy Plant Pro Kit

DNeasy Plant Pro Kit

 · DNA was isolated from 50 mg of plant material (4 replicates each) using the DNeasy Plant Pro, DNeasy Plant Mini and DNeasy PowerPlant Pro Kits. DNA yields were quantified with a Qubit Fluorometer. (A) Plants with high DNA content (grass and pine needles); (B) Plants with lower DNA content (old strawberry, mint or citrus (lemon) leaf).

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CTAB Protocol for the Isolation of DNA from Plant Tissues

CTAB Protocol for the Isolation of DNA from Plant Tissues

CTAB Protocol for Isolating DNA from Plant Tissues US and Canadian vistors, request a FREE SAMPLE of our CTAB based SYNERGY™ 2.0 Plant DNA Extraction Kit HERE . Isolating DNA from plant tissues can be very challenging as the biochemistry between divergent plant species can be extreme.

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Role of chemicals used in DNA extraction (Recombinant …

Role of chemicals used in DNA extraction (Recombinant …

 · Adding alcohol to a solution containing DNA is a simple way to obtain the pure DNA required, and colder temperatures slow down enzymes that can break down DNA, giving better extraction results. 5. Deionized water DNA get dissolve in water so we can keep it store in water to use DNA for the next experiments it is deionized you get rid of ions which can interfere with the extraction.

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Plant DNA Extraction Protocol

Plant DNA Extraction Protocol

Plant DNA Extraction Protocol Source: Protocol modified from Keb-Llanes et al. (2002) Plant Molecular Biology Reporter, 20: 299a−299e. Introduction Plant materials are among the most difficult for high quality DNA extractions. The key is to properly prepare

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Cambio

Cambio

Extraction & Purification - DNA: Plant Kits and reagents for DNA Isolation and purification from various sources including tissues, cells, microbial, plasmids, clinical, plant…

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DNA EXTRACTION METHODS

DNA EXTRACTION METHODS

Non-Organic DNA Extraction •Does not use organic reagents such as phenol or chloroform. •Digested proteins are removed by salting out with high concentrations of LiCl. •However, if salts are not adequately removed, problems could occur with the Dr.L

Get price
innuPREP Plant DNA Kit | Analytik Jena

innuPREP Plant DNA Kit | Analytik Jena

The innuPREP Plant DNA Kit has been specially developed for quickly and easily isolating DNA from an extremely wide variety of plant starting materials (such as leaves, stems, roots, flowers, etc.). In addition to efficient sample digestion, the extraction routine also includes a prefiltration step to effectively minimize unlysed plant components.

Get price
DNA EXTRACTION METHODS

DNA EXTRACTION METHODS

Non-Organic DNA Extraction •Does not use organic reagents such as phenol or chloroform. •Digested proteins are removed by salting out with high concentrations of LiCl. •However, if salts are not adequately removed, problems could occur with the Dr.L

Get price
Plant/Fungi DNA Isolation Kit | Sigma-Aldrich

Plant/Fungi DNA Isolation Kit | Sigma-Aldrich

Customer-Supplied Reagents and Equipment You must have the following in order to use the Plant/Fungi DNA Isolation Kit: Benchtop microcenrifuge 65 C Incubator Ice bath 96-100 % ethanol 70 % ethanolv Liquid nitrogen or any mechanical homogenizer

Get price
Veterinary Lab Workflows

Veterinary Lab Workflows

…Plant-PCR Kit includes novel buffer system that replaces the need for complicated extraction or purification steps, including freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction or column DNA purification. MPure-12 Plant DNA Extraction Kit is used with the MPure-12 instrument for extraction of genomic DNA from plant (leaf, seeds and spores) and fungal ...

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QuickExtract™ Plant DNA Extraction Solution, PCR-ready …

QuickExtract™ Plant DNA Extraction Solution, PCR-ready …

<B>Rapid and efficient extraction (no purification) of PCR-ready genomic DNA from plant leaves</B>The wide range of reagents are suitable for use with nucleic acids in transfection and transformation procedures, as well as cloning, sequencing, purification, and extraction. Use these reagents for isolating and purifying nucleic acids from biological material for use in further laboratory ...

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A simple and efficient genomic DNA extraction protocol for large scale genetic analyses of plant …

A simple and efficient genomic DNA extraction protocol for large scale genetic analyses of plant …

Asimpleandefficient genomic DNA extraction protocol for large scale genetic analyses of plant biological systems Kamirou Chabi Sikaa,b,c,TimnitKefelaa, Hubert Adoukonou-Sagbadja d,LeonardAhotone, Aliou Saidoue, Lamine Baba-Moussac, Lyla Jno Baptiste a, Simeon O. Kotconia,b,⁎,EmmaW.Gachomoa,b,⁎ ...

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Veterinary Lab Workflows

Veterinary Lab Workflows

…Plant-PCR Kit includes novel buffer system that replaces the need for complicated extraction or purification steps, including freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction or column DNA purification. MPure-12 Plant DNA Extraction Kit is used with the MPure-12 instrument for extraction of genomic DNA from plant (leaf, seeds and spores) and fungal ...

Get price
Plant DNA Extraction Protocol

Plant DNA Extraction Protocol

Plant DNA Extraction Protocol Source: Protocol modified from Keb-Llanes et al. (2002) Plant Molecular Biology Reporter, 20: 299a−299e. Introduction Plant materials are among the most difficult for high quality DNA extractions. The key is to properly prepare

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Genomic DNA Extraction

Genomic DNA Extraction

DNA extraction from a sample is a process of purifying the DNA. The sample can be tissue, plant or animal cells, blood, viral DNA or any other DNA containing the sample. The idea of extracting the DNA is quite basic: Disruption of the cell membrane (and cell wall in case of plant cells) to make the DNA exposed and then separate it from the rest of the cell debris.

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innuPREP Plant DNA Extraction Kit

innuPREP Plant DNA Extraction Kit

The innuPREP Plant DNA Kit has been specially developed for quickly and easily isolating DNA from an extremely wide variety of plant starting materials (suc Extraction A260 : A280 1.7 - 2.0 Starting material for extraction - Various plant materials e.g. wood

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Dna Extraction Kit Plant at Thomas Scientific

Dna Extraction Kit Plant at Thomas Scientific

…Plant-PCR Kit includes novel buffer system that replaces the need for complicated extraction or purification steps, including freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction or column DNA purification. MPure-12 Plant DNA Extraction Kit is used with the MPure-12 instrument for extraction of genomic DNA from plant (leaf, seeds and spores) and fungal ...

Get price
Enhanced Protocol for Isolation of Plant Genomic DNA

Enhanced Protocol for Isolation of Plant Genomic DNA

Several plant DNA extraction protocols have been used and compared to find the best protocol that isolates high-quality, amplifiable DNA. During the extraction, the aim is to lessen the effect of

Get price
How to set up a DNA extraction lab: A comprehensive …

How to set up a DNA extraction lab: A comprehensive …

Setting up a DNA extraction lab is quite difficult for the novice. However, it is not a big task yet we have to select all the utilities very carefully. which instruments are required, which chemicals are required and what is the SOP for different types of sample to proceed

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Equipment And Reagents For Dna Extraction Of Plant …

Equipment And Reagents For Dna Extraction Of Plant …

Equipment And Reagents For Dna Extraction Of Plant Materials Ops diagnostics synergy dna extraction platform now includes a 96 well plate format for isolating high quality plant dna in a timely and cost-effective manner without the use of hazardous chloroform building upon the …

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Plant/Fungi DNA Isolation Kit | Sigma-Aldrich

Plant/Fungi DNA Isolation Kit | Sigma-Aldrich

Customer-Supplied Reagents and Equipment You must have the following in order to use the Plant/Fungi DNA Isolation Kit: Benchtop microcenrifuge 65 C Incubator Ice bath 96-100 % ethanol 70 % ethanolv Liquid nitrogen or any mechanical homogenizer

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Equipment, materials, and reagents for DNA extraction and PCR

Equipment, materials, and reagents for DNA extraction and PCR

Equipment, materials, and reagents for DNA extraction and PCR A. Equipment • FastPrep FP-120 or -24 instrument (Qbiogene, Inc) or mini-bead beater (BioSpec) • PCR work station or enclosure • Vortex (any vendor) • Thermomixer (i.e: Eppendorf #5350

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An Efficient Multistrategy DNA Decontamination Procedure …

An Efficient Multistrategy DNA Decontamination Procedure …

 · The third major source of contaminating nucleic acids are reagents for DNA extraction and PCR. These reagents can be contaminated with DNA from various sources: human DNA, bacterial DNA and DNA from domestic animals. Plant-derived DNA could

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